Determination of urine neuron-specific enolase levels in neuroblastoma patients.
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Determination of urine neuron-specific enolase levels in neuroblastoma patients.
Urine levels of neuron-specific enolase were determined in 3 neuroblastoma patients (1 in an advanced state and 2 in remission), 25 control children, 37 control adults and 4 children with hematuria by means of the double-antibody inhibition radioimmunoassay specific to the gamma subunit of enolase isozymes. The levels of neuron-specific enolase mean +/- S.D. ng/creatinine mg in an advanced neur...
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This assay procedure for each of the two neuron-specific enolases (alpha gamma and gamma gamma) and the non-neuronal enolase (alpha alpha) in serum involves two steps: electrophoretic separation of the three isoenzymes--alpha alpha, alpha gamma, and gamma gamma--on cellulose acetate, and bioluminescence measurement of total enolase activity. From these data, the activity concentrations (U/L) of...
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BACKGROUND Serum levels of S-100B protein (S-100B) and neuron-specific enolase (NSE) are elevated after various cerebral injuries and are considered markers of central nervous system damage. In brain tumor patients, literature data on the prognostic value of serum S-100(B) and NSE levels are scarse and conflicting. PATIENTS AND METHODS We assessed serum S-100B and NSE levels in 20 consecutive...
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CLINICALCHEMISTRY, Vol. 34, No. 11, 1988 2375 trile, and applied the extract to a SepPak C18 cartridge (Waters Associates, Milford, MA) that had been washed sequentially with acetomtrile and water. To remove 24,25dihydroxy vitamin D, we washed the cartridge with water, then with methanol:water (7:3 by vol). 250HD was eluted with acetonitrile; the eluate was evaporated under nitrogen and the dri...
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ژورنال
عنوان ژورنال: The Tohoku Journal of Experimental Medicine
سال: 1985
ISSN: 0040-8727,1349-3329
DOI: 10.1620/tjem.147.429